TABLE 3.
Differential expression of the hDC inflammasome genes after 4 h and 24 h of coincubation with M. hominis PG21a
| Protein name |
t = 4 h |
t = 24 h |
||
|---|---|---|---|---|
| Fold change | P value | Fold change | P value | |
| Absent in melanoma 2 | 0.7 | 0.269 | 4.3 | 0.057 |
| B-cell CLL/lymphoma 2 | 4.4 | 0.036 | 20.7 | 0.034 |
| BCL2-like 1 | 1.4 | 0.100 | 3.4 | 0.059 |
| Baculoviral IAP repeat containing 2 | 1.2 | 0.577 | 3.5 | 0.039 |
| Baculoviral IAP repeat containing 3 | 2.1 | 0.708 | 34.7 | 0.029 |
| Caspase recruitment domain family, member 18 | Undetected | Undetected | Undetected | Undetected |
| Caspase recruitment domain family, member 6 | −1.7 | 0.309 | −1.7 | 0.155 |
| Caspase 1, apoptosis-related cysteine peptidase (interleukin 1β convertase) | 1.1 | 0.677 | 1.1 | 0.767 |
| Caspase 5, apoptosis-related cysteine peptidase | 3.1 | 0.019 | 5.8 | 0.030 |
| Caspase 8, apoptosis-related cysteine peptidase | −1.8 | 0.492 | −1.2 | 0.412 |
| Chemokine (C-C motif) ligand 2 | 1.1 | 0.975 | 80.9 | 0.010 |
| Chemokine (C-C motif) ligand 5 | 30.3 | 0.042 | 35.1 | 0.221 |
| Chemokine (C-C motif) ligand 7 | −1.1 | 0.720 | Undetected | Undetected |
| CD40 ligand | −4.3 | 0.401 | −2.1 | 0.205 |
| CASP8 and FADD-like apoptosis regulator | 1.7 | 0.604 | 5.9 | 0.024 |
| Conserved helix-loop-helix ubiquitous kinase | −1.1 | 0.332 | 1.6 | 0.467 |
| Class II major histocompatibility complex, transactivator | −1.1 | 0.938 | −4.3 | 0.024 |
| Cathepsin B | 1.0 | 0.999 | −2.8 | 0.011 |
| Chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity alpha) | 2.0 | 0.038 | 934.5 | 0.031 |
| Chemokine (C-X-C motif) ligand 2 | 1.0 | 0.540 | 265.2 | 0.054 |
| Fas (TNFRSF6)-associated via death domain | −2.0 | 0.254 | 1.0 | 0.978 |
| Heat shock protein 90kDa alpha (cytosolic), class A member 1 | −1.2 | 0.317 | −1.3 | 0.296 |
| Heat shock protein 90kDa alpha (cytosolic), class B member 1 | 1.0 | 0.905 | −1.9 | 0.150 |
| Heat shock protein 90kDa beta (Grp94), member 1 | −1.2 | 0.250 | −1.1 | 0.848 |
| Interferon beta 1 fibroblast | −2.4 | 0.570 | Undetected | Undetected |
| Interferon gamma | Undetected | Undetected | Undetected | Undetected |
| Inhibitor of kappa light polypeptide gene enhancer in B cells. kinase beta | 1.1 | 0.711 | 1.2 | 0.585 |
| Inhibitor of kappa light polypeptide gene enhancer in B cells. kinase gamma | −1.2 | 0.659 | 1.2 | 0.500 |
| Interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35) | Undetected | Undetected | Undetected | Undetected |
| Interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40) | 43.4 | 0.010 | 2,660.2 | 0.018 |
| Interleukin 18 (interferon-gamma-inducing factor) | −1.5 | 0.319 | 1.7 | 0.435 |
| Interleukin 1β | 8.0 | 0.040 | 285.5 | 0.035 |
| Interleukin 33 | Undetected | Undetected | Undetected | Undetected |
| Interleukin 6 (interferon beta 2) | −1.1 | 0.973 | 4,784.0 | 0.016 |
| Interleukin-1 receptor-associated kinase 1 | 1.4 | 0.482 | −1.3 | 0.385 |
| Interferon regulatory factor 1 | 1.8 | 0.184 | 8.8 | 0.040 |
| Interferon regulatory factor 2 | 1.2 | 0.811 | 2.7 | 0.046 |
| Mitogen-activated protein kinase kinase kinase 7 | −1.3 | 0.185 | −1.1 | 0.455 |
| Mitogen-activated protein kinase 1 | −1.2 | 0.729 | −1.2 | 0.299 |
| Mitogen-activated protein kinase 11 | 1.4 | 0.138 | 3.5 | 0.047 |
| Mitogen-activated protein kinase 12 | 2.0 | 0.105 | −1.9 | 0.202 |
| Mitogen-activated protein kinase 13 | 1.0 | 0.999 | 2.6 | 0.047 |
| Mitogen-activated protein kinase 3 | −1.5 | 0.623 | −3.8 | 0.035 |
| Mitogen-activated protein kinase 8 | 1.0 | 0.792 | 3.3 | 0.021 |
| Mitogen-activated protein kinase 9 | −1.2 | 0.523 | −2.9 | 0.038 |
| Mediterranean fever | 1.9 | 0.055 | −2.2 | 0.111 |
| Myeloid differentiation primary response gene (gene 88 product) | −1.4 | 0.630 | 1.3 | 0.112 |
| NLR family, apoptosis inhibitory protein | −2.7 | 0.478 | −12.2 | 0.026 |
| Nuclear factor of kappa light polypeptide gene enhancer in B cells 1 | 2.3 | 0.537 | 11.5 | 0.010 |
| Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor. alpha | 2.2 | 0.473 | 6.5 | 0.018 |
| Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor. beta | 1.8 | 0.139 | 1.7 | 0.073 |
| NLR family, CARD domain containing 4 | −1.1 | 0.782 | −2.3 | 0.069 |
| NLR family, CARD domain containing 5 | 1.0 | 0.822 | 1.9 | 0.166 |
| NLR family, pyrin domain containing 1 | 1.5 | 0.352 | 1.3 | 0.590 |
| NLR family, pyrin domain containing 12 | 1.9 | 0.284 | Undetected | Undetected |
| NLR family, pyrin domain containing 3 | −1.1 | 0.952 | −21.8 | 0.030 |
| NLR family, pyrin domain containing 4 | Undetected | Undetected | Undetected | Undetected |
| NLR family, pyrin domain containing 5 | Undetected | Undetected | Undetected | Undetected |
| NLR family, pyrin domain containing 6 | Undetected | Undetected | Undetected | Undetected |
| NLR family, pyrin domain containing 7b | 0.9 | 0.788 | 1.9 | 0.329 |
| NLR family, pyrin domain containing 9 | −1.3 | 0.719 | 1.3 | 0.414 |
| NLR family member X1 | −5.4 | 0.034 | −2.1 | 0.280 |
| Nucleotide-binding oligomerization domain containing 1 | −8.2 | 0.004 | −1.3 | 0.276 |
| Nucleotide-binding oligomerization domain containing 2 | −6.0 | 0.030 | −1.2 | 0.668 |
| Purinergic receptor P2X, ligand-gated ion channel, 7 | 18.6 | 0.070 | 7.2 | 0.064 |
| Pannexin 1 | −1.3 | 0.267 | 2.9 | 0.017 |
| Phosphoprotein enriched in astrocytes 15 | 1.1 | 0.665 | 2.0 | 0.233 |
| Proline-serine-threonine phosphatase interacting protein 1 | −1.9 | 0.352 | −13 | 0.029 |
| Prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) | 4.7 | 0.033 | 152.6 | 0.032 |
| PYD and CARD domain containing | −2.3 | 0.204 | −23.7 | 0.045 |
| PYD (pyrin domain) containing 1 | 1.5 | 0.507 | 3.2 | 0.920 |
| Renal tumor antigen | −1.3 | 0.038 | −1.3 | 0.314 |
| V-rel reticuloendotheliosis viral oncogene homolog A (avian) | 0.5 | 0.031 | 2.2 | 0.045 |
| Receptor-interacting serine-threonine kinase 2 | 2.1 | 0.579 | 9.5 | 0.023 |
| SGT1, suppressor of G2 allele of SKP1 (Saccharomyces cerevisiae) | −1.1 | 0.652 | 1.1 | 0.157 |
| TGF-beta activated kinase 1/MAP3K7 binding protein 1 | −2.6 | 0.490 | −1.6 | 0.038 |
| TGF-beta activated kinase 1/MAP3K7 binding protein 2 | −1.2 | 0.756 | 2.6 | 0.027 |
| Toll-interleukin 1 receptor (TIR) domain containing adaptor protein | −1.6 | 0.437 | −1.9 | 0.264 |
| Tumor necrosis factor | 2.4 | 0.489 | 7.6 | 0.110 |
| Tumor necrosis factor (ligand) superfamily, member 11 | Undetected | Undetected | Undetected | Undetected |
| Tumor necrosis factor (ligand) superfamily, member 14 | 4.0 | 0.054 | 1.4 | 0.350 |
| Tumor necrosis factor (ligand) superfamily, member 4 | 1.0 | 0.861 | 38.7 | 0.006 |
| TNF receptor-associated factor 6 | −1.2 | 0.140 | 1.6 | 0.121 |
| Thioredoxin interacting protein | −1.5 | 0.062 | −3.3 | 0.264 |
| X-linked inhibitor of apoptosis | 1.0 | 0.979 | 2.4 | 0.041 |
a
The lipoproteins for which significant fold changes (P value < 0.05) were observed are in boldface. Three independent biological replicates were performed for each time point.
b
The expression level of the NLRP7-coding gene, not present in the PCR Human Inflammasome (Qiagen) array, was determined by qRT-PCR.