FIG 2.

S. aureus growth in the presence of desleucyl-oritavancin. (a) Growth curve of S. aureus (ATCC 6538P) in the presence of des-Ori, monitored by measuring the optical density at 600 nm (OD₆₀₀). des-Ori was added to S. aureus at an OD₆₀₀ of 0.6, and the cells were harvested after 100 min of growth in the presence of des-Ori. Cell walls of S. aureus were synthesized in the presence of des-Ori at concentrations of 0, 1, 5, 10, 15, and 20 μg/ml during mid-exponential growth (dashed lines). (b and c) Following the des-Ori addition, S. aureus exhibited concentration-dependent growth inhibition as determined by (i) OD₆₀₀ measurements after 100 min of growth in the presence of des-Ori (b) and (ii) the time delay to the point of inflection, which indicates the recovery of growth (Fig. 2c).