TABLE 2.
Persistence of infectious LPAI H7N2 virus under cat shelter environmental conditions
| Virusa | Titerb (EID50/ml) | Virus detection at the following times postincubationc (h): |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 0 | 0.5 | 1 | 1.5 | 2 | 2.5 | 3 | 3.5 | 4 | ||
| NY/108 | 1.34 × 104 | + | + | + | + | − | − | − | − | − |
| 3.11 × 104 | + | + | + | + | + | + | + | − | − | |
| 3.11 × 104 | + | + | + | + | + | + | + | + | + | |
| NY/107 | 2.24 × 103 | + | + | − | − | − | − | − | − | − |
| 2.65 × 103 | + | + | + | + | + | + | + | − | − | |
| 1.37 × 104 | + | + | + | + | − | − | − | − | − | |
| Tky/VA | 2.65 × 104 | + | + | + | + | + | − | − | − | − |
| 6.22 × 105 | + | + | + | + | + | + | + | + | + | |
| 1.37 × 104 | + | + | + | + | + | + | + | + | + | |
a
For each virus, each row represents an independent experiment.
b
Baseline titer at time zero postincubation at 20°C and 50% relative humidity.
c
Virus (5 μl) was deposited in individual wells of a 96-well plate and allowed to dry. At the indicated times, 200 μl of PBS was added to each well and the contents of the wells were pipetted for ∼10 s to rehydrate and resuspend any viral material present and frozen at −80°C until titration in eggs. +, infectious virus was detected in >50% of the wells (limit of detection, 6.3 EID50/ml); −, no infections virus was detected in >50% of the wells.