FIG 1.

Identification of human proteins that associate with arenavirus nucleoproteins. (A) Overview of experimental approach to identify human cellular protein partners of arenavirus nucleoproteins in the context of infected cells. A549 or HEK 293T cells were infected with either JUNV Candid #1 or LCMV Armstrong 53b or left uninfected (mock). Cells were lysed, and NP was immunoprecipitated with monoclonal antibodies. The immunoprecipitated fractions were separated by SDS-PAGE, and lanes were cut into multiple slices. Proteins were digested with trypsin, peptides were extracted from each slice, and proteins were identified by tandem mass spectrometry. (B to E) Representative Coomassie blue-stained gels of immunoprecipitated NP from JUNV- or mock-infected A549 cells (B), LCMV- or mock-infected A549 cells (C), JUNV- or mock-infected HEK 293T cells (D), or LCMV- or mock-infected HEK 293T cells (E). Immunoprecipitated NP is indicated by an arrowhead in each gel. Background bands composed of IgG heavy and light chains are denoted with asterisks. (F) Venn diagram representing the numbers of host proteins interacting with JUNV NP, LCMV NP, or both. (G) Venn diagram representing the numbers of host protein partners (of either JUNV or LCMV NP) identified in A549 cells, HEK 293T cells, or both. The Coomassie blue-stained gels shown in panels B to E are representative of 2 independent experiments.