TABLE 1.

Gene expression during i.d. virus infection as detected by RT-qPCR

Gene	Expressiona
	Day 1 p.i.			Day 3 p.i.			Day 5 p.i.
	vK1L	vΔK1L	vK1Lrev	vK1L	vΔK1L	vK1Lrev	vK1L	vΔK1L	vK1Lrev
Chemokines
Ccl4	0.47	0.33	0.54	1.90	0.62	1.98	55.97	4.51	49.82
Cxcl2	0.48	0.30	0.53	1.44	0.60	1.15	10.41	1.99	8.83
Cxcl5	0.17	0.14	0.18	2.71	0.44	8.92	29.76	5.62	39.96
Ccl2	0.64	0.80	0.66	4.28	2.00	8.06	12.91	6.30	16.27
Ccl5	0.73	0.69	0.75	0.54	0.49	0.89	7.41	1.84	6.84
Cxcl9	1.94	0.87	1.07	3.41	0.95	8.22	307.96	87.92	360.41
Cxcl10	0.91	0.52	0.64	9.03	1.02	22.95	302.86	193.49	419.48
Cxcl1	2.68	1.52	2.16	18.23	10.46	26.78	13.95	16.10	20.90
Cytokines
Il1b	0.35	0.24	0.45	1.57	0.45	1.09	12.47	2.67	11.38
Il6	0.46	0.25	0.44	7.11	1.73	19.65	49.03	13.44	63.77
Il10	0.54	0.42	0.59	2.28	0.94	4.12	9.76	2.93	12.76
Il7	1.19	0.96	0.98	0.70	0.99	0.52	0.59	0.73	0.61
Tnf	1.04	0.89	0.91	1.16	0.94	0.97	0.86	0.97	0.78
IFNs
Ifng	1.45	0.71	1.01	3.40	1.02	7.81	247.88	32.21	306.88
Ifna4	1.41	1.40	1.29	0.60	0.85	0.85	0.19	0.51	0.22
Ifnb	1.67	1.41	1.42	1.23	1.02	2.24	0.91	1.20	1.17
ISGsb
Isg15	0.83	0.55	0.87	3.39	0.94	9.27	104.70	36.81	115.28
Rnasel	1.07	0.91	0.91	0.95	0.92	1.23	3.38	1.52	3.82
Mx1	0.50	0.51	0.53	3.55	0.99	9.65	116.42	68.74	174.37
Eif2ak2	1.12	0.95	1.10	1.29	1.07	1.56	2.64	1.91	3.02
Keratinocyte stress response
Krt6a	0.40	0.34	0.45	1.61	0.54	2.12	32.94	13.01	37.82
Krt16	0.52	0.47	0.52	2.21	0.87	2.84	15.39	6.54	18.60
NF-κB pathway
Nfkb2	1.17	1.04	1.02	1.08	1.12	0.93	0.52	0.93	0.57
Nfkbia	1.14	0.88	1.05	0.87	1.02	0.71	0.69	0.83	0.72

^aThe data represent the average fold expression level of each gene (2^−ΔΔCT) compared to that in PBS-inoculated ears that were harvested on the same day p.i. (n = 4 ears per group). The underlined values indicate a statistically significant difference compared to PBS-inoculated mice (P < 0.05). The boldface values indicate statistically significant differences between mice inoculated with vK1L and mice inoculated with vΔK1L (P < 0.05).

^bISG, interferon-stimulated gene.