TABLE 2.
Primers used to generate the viral constructs
| Primer name | Sequence (5′–3′)a |
|---|---|
| JFH1-7453-RsrIIF | ATCCGGCGGTCCGACGT |
| JFH1-7525-MluR | AGGCTCACGCGTGAGGGGGGGCATAGAGGAGGC |
| JFH1-7525-MluF | CTCACGCGTGAGCCTGGAGATCCGGACCTGGAGTC |
| JFH1-7796-BsrGIR | TCTTTGATGTTGTACAGTACACCTTG |
| NS5A-Rluc-HF | GCCCCCCCTCACGCGTATGGCTTCCAAGGTGTACG |
| NS5A-Rluc-HR | CTCCAGGCTCACGCGTCTGCTCGTTCTTCAGCACGCGC |
| 2a-78F | CTAGCCATGGCGTTAGTATGAGTGT |
| 2a-1731R | ATGACCTCGGGGACGCG |
| 2a-E1A4F | CAGGTGAAGAATTCCAGTGGCCTCTACCATGTGACCAATGACTGC |
| 2a-E1A4R | GCAGTCATTGGTCACATGGTAGAGGCCACTGGAATTCTTCACCTG |
| ApoE3-KasIF | GAGGGCGCCGAGCGCGG |
| ApoE-SG-HAR | GTATGGGTATCCTCCTCTTCCTCCTGAGTGATTGTCGCTGGGCACA |
| ApoE dCT-SG-HAR | GTATGGGTATCCTCCTCTTCCTCCTGAGGCCTGCAGGCGTATCTG |
| HA-SacI-XbaIR | GGTCTAGAGCTCAAGCGTAATCTGGAACATCGTATGGGTATCCTCCTCTTCCTCCTG |
| Hbactin-KpnIF | AGTGGTACCATGGATGATGATATCGCCGCG |
| Hbactin-XbaIR | AGTTCTAGACTAGAAGCATTTGCGGTGGACG |
| ApoE129-g20F | CACCGCCAGTGCCAGTTCCCAGCGC |
| ApoE129-g20R | AAACGCGCTGGGAACTGGCACTGGC |
| H77-741-BglIIF | AATAGATCTGCCACCATGGGGTACATACCGCTCGTCGGCG |
| H77-2579-XbaIR | GAATCTAGATCACGCCTCCGCTTGGGATATGAGtaac |
| JFH1-740- BglIIF | AATAGATCTGCCACCATGGGGTACATCCCCGTCGTAGGCG |
| JFH1-2590-XbaIR | GAATCTAGATCATGCTTCGGCCTGGCCCAACAAGATG |
| S52-739-BglIIF | AATAGATCTGCCACCATGGGGTACATCCCGCTCGTCGGCG |
| S52-2592-XbaIR | GAATCTAGATCATGCTTCTGCTTGTGATACCATCAGCATC |
| ED43-740-BglIIF | AATAGATCTGCCACCATGGGATACATCCCGCTCGTAGGCG |
| ED43-2575-XbaIR | GAATCTAGATCACGCCTCAACTTGACTTACCATAAACATCATCC |
| SA1-739-BglIIF | AATAGATCTGCCACCATGGGGTACATCCCGCTCGTAGGCG |
| SA1-2577-XbaIR | GAATCTAGATCATGCTTCTGCCTGACAAACTAGAAGC |
| CP16-740-BglIIF | AATAGATCTGCCACCATGGGGTACATCCCAGTCCTAGGAG |
| CP16-2584-XbaIR | GAATCTAGATCAGGCCTCCGCTTGGCCCACCAGGAAC |
| H77-171-NheF | TTGGCTAGCATGGGTTGCTCTTTCTCTATCTTCCTTCTGG |
| H77-383-BglIIR | TTGAGATCTTACGCGTCGACGCCGGCAAATAG |
| H77-384-NheF | TTGGCTAGCATGGAAACCCACGTCACCGGGGGAAATG |
| H77-746-BglIIR | TTGAGATCTTACGCCTCCGCTTGGGATATGAG |
a
The restriction sites used to facilitate cloning are underlined.