FIG 4.

Rescue of AKAV and SBV infectivities by adding back the EXT2 gene to EXT2-KO cells. (A) Flow cytometric analysis of adding back the EXT2 gene in EXT2-KO-1 HmLu-1 cells. Cells were labeled with anti-heparan sulfate mouse monoclonal antibody (10E4) and analyzed by flow cytometry (FACSVerse; BD Biosciences). (B) Infectivities of AKAV and SBV in cells with EXT2 added back. The cells were infected with AKAV(OBE-1), AKAV(Iriki), or SBV. Cells were stained for AKAV or SBV antigen, and positive cells were counted under a fluorescence microscope. Results are expressed as percentages relative to the number of wild-type cells. The data are reported as the mean value with standard deviations for three independent experiments.