FIG 2.

Primary piRNA-like small RNA production from ALFE1 to ALFE3 and AEFE1. The size distribution (top) and profiles (middle) of small RNAs mapped to ALFE1 to ALFE3 in A. albopictus mosquitoes and AEFE1 in A. aegypti mosquitoes not infected with CHIKV (A) or infected with CHIKV (B) are shown. Orange and blue bars, positive- and negative-stranded reads, respectively. For the small RNA profiles, the x axis represents the nucleotide position on the ALFE1-, ALFE2-, ALFE3-, or AEFE1-containing contig; the y axis shows the number of reads for each nucleotide position; gray lines, uncovered regions. (Bottom) Relative nucleotide frequency per position of the 28-nucleotide (nt) ALFE1-, ALFE2-, ALFE3-, or AEFE1-derived small RNAs shown as a heat map. The intensity varies in correlation with frequency.