FIG 4.

DENV1 and -3 infections have no detectable impact on AEFE1 transcript expression in vivo. The AEFE1 transcription level was analyzed by use of the DESeq2 package and a transcriptome data set from A. aegypti mosquitoes infected or not infected (Mock) with DENV1 (A) and A. aegypti mosquitoes infected with DENV1 or -3 (B) at the indicated time points. The average number of reads was calculated from several individual libraries: 7 and 17 libraries for mock- and DENV1-infected mosquitoes, respectively, at 24 h postinfection (hpi); 6 and 17 libraries for mock- and DENV1-infected mosquitoes infected, respectively, at 96 h postinfection; and 3 for each library for DENV1- or DENV3-infected mosquitoes at both time points. Statistical significance was determined by use of the DESeq2 package. ns, no significant difference (adjusted P value, >0.05).