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. 2017 Jul 12;91(15):e00571-17. doi: 10.1128/JVI.00571-17

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Copyright © 2017 Suzuki et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 9 — Phylogenetic relationships among ALFE1 and insect-specific flaviviruses. A maximum likelihood tree was generated using the Fast likelihood-based approach. ALFE1 DNA from individual mosquitoes of the A. aegypti Cameroon, French Guiana, and Thailand strains was sequenced. The tree was generated with Aedes flavivirus (GenBank accession no. AB488408.1), cell-fusing agent virus (GenBank accession no. NC_001564.2) and Kamiti River virus (GenBank accession no. AY149905.1) as ISFs and DENV1 (GenBank accession no. KX225493.1), DENV2 (GenBank accession no. AY858035.2), DENV3 (GenBank accession no. AY858047.2), DENV4 (GenBank accession no. KU523872.1), ZIKV (GenBank accession no. KU955595.1), West Nile virus (GenBank accession no. M12294.2), Yellow fever virus (GenBank accession no. NC_002031.1), and Tamana bat virus (GenBank accession no. AF285080.1) and rooted using Tamana bat virus. The scale bar indicates the number of nucleotide substitutions per site. Node values represent Shimodaira-Hasegawa (SH)-like branch support (only values of >0.7 are shown).