TABLE 2.
Primer pairs for PCR and RT-PCR
| Target | Name of primer | Sequence |
|---|---|---|
| ALFE1 flanking region | ALFE1_-100F | CCCTGTCACACAAGCTTGGAG |
| ALFE1_ + 99R | CCTACCATGGAGGGTGTTCTGTTTG | |
| Full-length ALFE1 | ALFE1_F | ATGGTGGTCGTCTTCACTATGTACATAC |
| ALFE1_R | CTAATTCTTGCTGCAAGCTGAGTTCTG | |
| ALFE1 without first 200 bp | ALFE1_200 | GCACAAATAAACCATGCCTAATCTGCG |
| ALFE1_R | CTAATTCTTGCTGCAAGCTGAGTTCTG | |
| ALFE1 NS2 region | ALFE1_NS2_F | AGATCGGATTTTCACCCAGCGAAC |
| ALFE1_NS2_R | TCTACAATGCTCGTTAAGTTTTAAGCG | |
| Full-length ALFE2 | ALFE2_F | ACGGCAGTAATGACCTGTGCTG |
| ALFE2_R | CCCCAATACTTTATCTCCGTGCCG | |
| Full-length ALFE3 | ALFE3_F | CGAGATCACCGCGCGAATCC |
| ALFE3_R | GTGGCTAGATTGTAGCCGCGAG | |
| Full-length AEFE1 | AEFE1_F | TCTGCCAGGGACGTGTACATG |
| AEFE1_R | GTTTTCGTTGTTTGGTGTGATGGATGG | |
| First 1.2 kbp of AEFE1 | AEFE1_F | TCTGCCAGGGACGTGTACATG |
| AEFE1_1200_R | GAAAGTGAAGGGCTACTCGAAGCTG | |
| 18S rRNA | 18S_F | GGTCGGCGCGGTCGTAGTGTGG |
| 18S_R | TCCTGGT GGTGCCCTTCCGTCAAT | |
| Actin | Actin_F | AAGGCTAACCGYGAGAAGATGAC |
| Actin_R | GATTGGGACAGTGTGGGAGAC | |
| ALFE4 | ALFE_NS4_F | TTGTCCATTGAGATGTATATCAATACCCG |
| ALFE3_R | GTGGCTAGATTGTAGCCGCGAG | |
| ALFE6 | ALFE6_F | AAAGCTTTACTACTCGAAAACTCCC |
| ALFE7_R | TCCTTTATGTACAGATTTGTGAATGCG | |
| ALFE7 | ALFE7_F | TCACTCCGTGGAGCTGGATG |
| ALFE7_R | TCTTTCTTAAAATTGATACCTTCCATTGATC | |
| ALFE8 | ALFE8_F | CTAAAACATTATGAGTGAAAATGGCAG |
| ALFE8_R | GCTCTTCCTCATCTTGCAAGTCG | |
| ALFE9 | ALFE9_F | AGATGAGAACCTTGAAGACACCCTTAG |
| ALFE9_R | CAGTCTGTGGTAATGTTGTTGGCG | |
| ALFE10 | ALFE10_F | AGATGAGAACCTTGAAGACACCC |
| ALFE10_R | CAAATCACCAGCGAAGGCTTTC |