FIG 11.

Histologic evaluation of the liver and cholangiograms performed 12 days after infection with RRV and RRV^VP4-R446G. (A) Hematoxylin and eosin staining of the liver after infection with RRV shows severe inflammation with an accumulation of inflammatory cells. (B) In contrast, mice injected with RRV^VP4-R446G had mild inflammation. Magnification, ×10. (C and D) Cholangiograms demonstrating injection of methylene blue dye into the gallbladders of mice at 12 to 14 days postinoculation. The cholangiograms show the presence of biliary obstruction with RRV and the absence of biliary obstruction with RRV^VP4-R446G. There were 7 mice in the RRV group and 8 in the RRV^VP4-R446G group.