FIG 7.

Viral binding and replication in murine cholangiocytes. (A) Percentages of RRV, RRV^VP4-XbaI, and RRV^VP4-R446G binding to murine cholangiocytes at 4°C. The quantity of bound virus was measured using FFA. (B) Quantification of infectious rotavirus strains in murine cholangiocytes at 24 and 48 h postinfection, measured using FFA. For both sets of experiments, values (n = 3) are expressed as mean FFU per milliliter, with standard errors; each assay was repeated three times. (C) Cholangiocytes were pretreated with 1 mM synthetic peptides TRTRVSRLY, DGEA, and GHRP, followed by infection with RRV or RRV^VP4-R446G. The DGEA peptide was able to inhibit binding of both strains, while TRTRVSRLY was able to block only WT RRV binding. *, P < 0.05.