TABLE 2.
Primers used for this study
| Primer no. | Nucleotide sequence (5′–3′)a | Comment |
|---|---|---|
| 1 | CAGCATCCCGGGTAATACGACTCACTATAGGCTATAAAATGGCTTCGCTCAT | SmaI site |
| 2 | ATCGACCGCGGTCACATCCTCTGGAAATTGCTTAC | SacII site |
| 3 | ATCGTTACCAACGGATTTTGTAATATCTAGATATGAAGTAATTAAAGAGAATTC | |
| 4 | GAATTCTCTTTAATTACTTCATATCTAGATATTACAAAATCCGTTGGTAACGAT | |
| 5 | CACCAGAACTAGAGTTAGTGGATTGTATGGGTTACCTGC | R-to-G change |
| 6 | GCAGGTAACCCATACAATCCACTAACTCTAGTTCTGGTG | |
| 7 | CATATACCGTTGACTTATCTGATG | RRV VP4 |
| 8 | TAATGGTCCGTATTGTGAATAGC | |
| 9 | GGGGTCCTGGTGAAACTAATG | Rotavirus VP4 |
| 10 | AAGCGCCAATGGAACAATG | |
| 11 | ATACGGACCATTACAATCTACTCCA | RRV462 |
| 12 | ATTCTTTTCCATTATTTGGGTTAGC | RRV1381 |
a
The restriction enzyme site for XbaI is underlined.