FIG 1.

Recombinant viruses used in this study. (A) The pUL25 open reading frames (580 amino acids) from wild-type KOS and pUL25 recombinant viruses are shown, along with the location of the GFP insertion and the C-terminal three-amino-acid deletion. The virus names are indicated on the left of each construct. The amino acid numbers above each construct indicate protein lengths with the pUL25 C-terminal amino acids indicated. (B) The titers of viruses grown on Vero cells (KOS, vFH422) or UL25-complementing, 8-1 cells (vFH518, vFH589) were determined on the two cell lines. (C) KOS and vFH518 were grown on Vero cells, and their titers on Vero or 8-1 cells were determined. The plates were stained with crystal violet 3 days (KOS) or 5 days (vFH518) postplating.