FIG 10.

Compromised antiviral recognition of pUL25 mutant HSV in differentiated THP1 cell lines. A dose-response antiviral activation assay with KOS, vFH422, vFH518, and vFH589 viral strains was carried out in human macrophage THP1 cells. Cells were incubated with the indicated genome equivalents (500, 1,500, or 4,500 genomes/cell) of each virus, and total cell lysates were harvested 4 h postinfection. Mock samples were treated with medium only as a negative control, and cells exposed to Ad5 and Ad35 viruses provide weak and strong positive controls for activation of the cGAS/STING antiviral cascade in THP1 cells (31). Western blot analysis of each lysate was carried out and probed with the indicated antibodies as described in Materials and Methods.