FIG 3.

Analysis of virions isolated from Vero cells infected with KOS or the UL25 mutant virus. (A) Vero cells infected with the indicated viruses at an MOI of 5 PFU per cell were harvested at 18 h postinfection, and virions were isolated from the medium on 20 to 60% sucrose gradients. (B) Equivalent amounts of virions were separated by SDS-PAGE, and the proteins were detected by Coomassie blue staining. The positions of the HSV virions proteins are indicated on the right. (C) Immunoblot analysis of virions with UL25 and GFP antibodies. The positions of the pUL25 and pUL25-GFP proteins are indicated on the right. Molecular mass standards are given in kilodaltons.