Figure 2.

Amplification plots of real-time PCR assays. C. trachomatis PCR (A–D), pan-Chlamydia PCR (E–H), and new pan-Chlamydia/C. felis duplex PCR assay (I–L) on the conjunctival swab from the patient (A,E,I), conjunctival swab from the cat (B,F,J), and throat swab from the cat (C,G,K). (D,H) Positive controls. Results (+, positive; −, negative) are given within the panels for internal control (IC, black), C. trachomatis (Ct, green), pan-Chlamydia PCR (Cs, blue), and the pan-Chlamydia/C. felis duplex PCR (Cs/Cf) containing both the genus-specific (Cs, blue) and the C. felis-specific probe (Cf, red). (L) Specificity controls of the new pan-Chlamydia/C. felis duplex PCR showing positive results with the genus-specific probe (green, black, and blue), but no cross-reactivity of the C. felis-specific probe (red). Three reactions are combined in one panel: DNA from C. psittaci (Cps, cell culture, 10⁵ infection forming units per PCR), C. pneumoniae (Cpn, cell culture, 2 × 10⁴ genomic copies per PCR), and C. trachomatis (Ct, from urethral swab specimen, 4 × 10³ infection forming units per PCR).