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. 2017 Jul 17;4:105. doi: 10.3389/fmed.2017.00105

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Copyright © 2017 Wons, Meiller, Bergua, Bogdan and Geißdörfer.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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23S rRNA gene fragment as target for the pan-Chlamydia PCR. The primers (TQF and TQR) are highlighted in yellow, and the pan-Chlamydia probe (TQP) in green. The DNA sequence used as C. felis-specific probe (this study) is underlined. Note that despite the overlap of the two probes, there is no interference in detection because they bind to complementary DNA strands. 44 bp of conserved sequences are blanked out (marked by #). GenBank accession numbers for the aligned sequences are: C. felis (NR_076260, bp 516–646), C. trachomatis (NR_103960), C. psittaci (NR_102574), C. pneumoniae (NR_076161), C. avium (NR_121988), C. abortus (NR_077001), C. caviae (NR_076195), C. pecorum (NR_103180), C. muridarum (NR_076163).