FIG. 7.
STAT1 and STAT3 are involved in HIV-1 replication. MDM were treated with 100 nM Fludarabine (STAT1 inhibitor) or 50 nM LLL12 (STAT3 inhibitor) 24 h before infection and total DNA was extracted on PID 1. Total (A) or integrated (B) HIV-1 proviral genomes were measured using qPCR, normalized to ApoB, and compared with medium alone (Fludarabine control) or DMSO (LLL12 control). Bars represent the mean ± SEM. (C) MDM treated with Fludarabine or LLL12, 24 h pre- and postinfection, with single-cycle luciferase reporter HIV-1JRFL were lysed on PID 4 and luciferase activity was measured. Bars represent mean ± SEM relative light units (RLU); **p < .01 using paired T-test. At least three donors were used in the experiment.