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. 1980 Sep;29(3):1028–1033. doi: 10.1128/iai.29.3.1028-1033.1980

Production and properties of heat-stable extracellular hemolysin from Pseudomonas aeruginosa.

M K Johnson, D Boese-Marrazzo
PMCID: PMC551234  PMID: 6776058

Abstract

Of 12 strains of Pseudomonas aeruginosa, 10 were found to produce heat-stable extracellular hemolysin in highly aerated peptone broth supplemented with glycerol, fructose, or mannitol. Glucose supported good hemolysin production only in medium that was highly buffered. The yield of both cells and hemolysin was lower with organic acids as supplement. Growth-limiting phosphate concentrations produced maximum hemolysin levels. Purified hemolysin preparations contained two hemolytic glycolipids. The kinetics of hemolysis at various levels of purified lysin and the effects of variation in lysin and erythrocyte concentration are described.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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