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Representative pic of methylation-specific polymerase chain reaction showing different patterns of p15 gene methylation in APL patients. Lane 1, molecular weight markers (MW, 50 bp); lanes 2 and 3, unmethylated p15 gene (U, 154 bp); lanes 4 and 5, partial methylated p15 showing both 154 and 148 bp bands (U/M); lanes 6 and 7, methylated p15 gene (M, 148 bp); lane 8, positive control for methylated DNA (PC, 148 bp); lane 9, negative control without template DNA (NC).
