Figure 1.

Human choriodecidua harbors B cells that are dysregulated in PTL. (a,b) Frequency of CD19⁺ B cells in CD45⁺ cells in choriodecidua of women with TL (n = 21) or PTL (n = 12). (c) Calculated numbers of CD19⁺ B cells recovered from choriodecidual tissues of women with TL (n = 21) or PTL (n = 12). (d) Expression of CD20, CD22, CD23, CD27, CD38, CD138, IgM, IgD, CCR7 and BCMA on CD19⁺ B cells in peripheral blood (PB) of two nonpregnant healthy donors (black histogram) and choriodecidual tissues of women with TL (blue histogram) or PTL (red histogram). Shaded histograms indicate the staining of CD19⁺ B cells in the respective tissues using isotype control antibodies. The results summarize the profiles of 20 healthy blood donors, 16 women with TL and 12 women with PTL. (e,f) CD43 and CD27 expression on CD19⁺CD20⁺CD70⁻ B cells and the percentage of CD19⁺CD20⁺CD70⁻CD43⁺CD27⁺ cells in CD19⁺CD20⁺ B cells in choriodecidual tissues of women with TL (n = 15) or PTL (n = 12). (g) Calculated total numbers of CD19⁺CD20⁺CD70⁻CD43⁺CD27⁺ cells recovered from choriodecidual tissues of women with TL (n = 15) or PTL (n = 12). (h,i) CD24 and CD38 expression on CD19⁺ B cells and the frequency of CD24⁻CD38^hi PCs in CD19⁺ B cells in choriodecidual tissues of women with TL (n = 14) or PTL (n = 12). (j) Calculated total numbers of CD24⁻CD38^hi PCs recovered from choriodecidual tissues of women with TL (n = 14) or PTL (n = 12). (k,l) Frequency of IL-10⁺ B cells in CD19⁺ B cells in choriodecidual tissues of women with spontaneous TL (n = 8) or spontaneous PTL (n = 7). (m) Calculated total numbers of IL-10⁺ B cells recovered from choriodecidual tissues of women with TL (n = 8) or PTL (n = 7). **P < 0.01, ***P < 0.001, by two-tailed t test (b,c,g,i,j,l,m) or two-tailed Mann–Whitney U test (f,i).