The number of Iba1+ phagocytic cells at PN2 (a), Iba1+ cells with CD68+/DAPI+ phagocytic cups (b), the number of Iba1+ cells phagocytizing BrdU+ cells (c), the number of Iba1+ cells phagocytizing Sox2+ progenitor cells (d), the total number of Iba1+ phagocytic microglia at E20 (e), and summary of phagocytic targets (f). There were significantly more phagocytic microglia in females compared to males and females treated with estradiol (E2) (a). There were significantly more CD68+ phagocytic microglia in females compared to males (b). There were no differences in the number of BrdU+ cells phagocytized across groups (c). There were more microglia phagocytizing Sox2+ cells in females than in males (d). There was no sex difference in phagocytic microglia at E20 (e). * p < 0.05, ** p < 0.01.