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. 2017 Jul 17;8:806. doi: 10.3389/fimmu.2017.00806

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Copyright © 2017 Theron, Bentley, Nagel, Manchester, Gerg, Schindler, Silva, Ecabert, Teixeira, Perret and Reis.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Detection of a dose-dependent Lip10 intracellular accumulation in professional antigen-presenting cell (APC) subsets. (A) Representative gating scheme used for Lip10 detection in immune subtypes with a flow cytometry-based assay. (B) Lip10 accumulation [p10 median fluorescence intensity (MFI)] in different immune subtypes was assessed by flow cytometry after in vitro incubation of PBMC with a serial titration of RO5459072. Data represent five individual healthy volunteers, identified by identical individual colored lines and dots across graphs. Curve fit was determined by non-linear regression using a variable slope model with GraphPad Prism software.