Table 1.
Strains and plasmids used in this study.
| Strains or plasmids | Description | Reference |
|---|---|---|
| Methanococcus maripaludis strains | ||
| Mm900 | Mc. maripaludis S2 Δhpt, wild-type strain in this study | Moore and Leigh, 2005 |
| ΔearA | Mm900 ΔearAMma | Ding et al., 2016b |
| ΔearA-sp | A spontaneous mutant derived from ΔearA in which the transcription of flaB2 was restored | This study |
| Δ3A | A mutant created from ΔearA in which three adenines were deleted from the BRE region of the fla promoter | This study |
| Escherichia coli strains | ||
| TOP10 | F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(StrR) endA1 aaa- | Invitrogen |
| Plasmids | ||
| pCRPrtNeo | hmv promoter-hpt fusion plus Neor cassette in pCR2.1Topo; Ampr | Moore and Leigh, 2005 |
| pKJ1273 | pCRPrtNeo containing ∼2 kb region from fla promoter in which three adenines in the BRE region were deleted | This study |