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. 2017 Jul 17;8:1329. doi: 10.3389/fmicb.2017.01329

Table 2.

Primers used in this study.

Primers Sequence Restriction site incorporated (underlined)
Promoter-substitution primers
P-fus-F AGTCGGATCCATACATCAGTTTGACAGGAC BamHI
P-fus-R GACTGGATCCCAGCAAATGATGCATTAACG BamHI
Sequencing primers for promoter-substitution mutant screening
P1-For TTTATAGATTCTGGATGTTCAAATGC
P1-Rev ATCAAGGTACCAATTCCAGAAGC
earA-seq-F TGGATACGGTAAGTTCCATCG
earA-seq-R CAACTTCGAGAATAGTGTCTCC
qRT-PCR primers
B2-qRT-PCR-For GCTGCAATAGACATGAATCAGG
B2-qRT-PCR-Rev GACCAGTTTACAGTTGTAGTGTTG
slp-qRT-PCR-For GGTACTGAAGCATACGAAGGAG
slp-qRT-PCR-Rev GCTACAACTTTACCGTCTTTTAAGAG