Table 2.
Primers | Sequence | Restriction site incorporated (underlined) |
---|---|---|
Promoter-substitution primers | ||
P-fus-F | AGTCGGATCCATACATCAGTTTGACAGGAC | BamHI |
P-fus-R | GACTGGATCCCAGCAAATGATGCATTAACG | BamHI |
Sequencing primers for promoter-substitution mutant screening | ||
P1-For | TTTATAGATTCTGGATGTTCAAATGC | |
P1-Rev | ATCAAGGTACCAATTCCAGAAGC | |
earA-seq-F | TGGATACGGTAAGTTCCATCG | |
earA-seq-R | CAACTTCGAGAATAGTGTCTCC | |
qRT-PCR primers | ||
B2-qRT-PCR-For | GCTGCAATAGACATGAATCAGG | |
B2-qRT-PCR-Rev | GACCAGTTTACAGTTGTAGTGTTG | |
slp-qRT-PCR-For | GGTACTGAAGCATACGAAGGAG | |
slp-qRT-PCR-Rev | GCTACAACTTTACCGTCTTTTAAGAG |