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. Author manuscript; available in PMC: 2017 Jul 17.
Published in final edited form as: J Mol Biol. 2010 Jun 19;401(3):334–349. doi: 10.1016/j.jmb.2010.06.032

Figure 6.

Figure 6

Deficiencies in rna15-R87D are not limited to the GAL7 transcript and are due to an inability to form a stable RNP poly-protein processing complex in vivo. A) Coupled cleavage/polyadenylation assay using the same conditions as Figure 5A but with precursor containing the CYC1 poly(A) site. B) The rna15-R87D mutation weakens the association of Rna15 with GAL7-1 precursor incubated with extract. Processing reactions were incubated at 30°C for 30 min, and RNAs immunoprecipitated using Rna15p antibody (+) or preimmune serum (-). “Input” represents an aliquot of the reaction taken before immunoprecipitation, and “Pellet” indicates RNAs that are pulled down with the antibodies.