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. 2017 Jun 5;8:15564. doi: 10.1038/ncomms15564

Figure 2. POP2 inhibits cytokine production in mouse macrophages.

Figure 2

(a–c) Mean level±s.d. of TNF and IL-6 measured from culture supernatants of (a) BMDM, (b) peritoneal macrophages or (c) splenic macrophages from POP2Tg (n=4) and LMC (n=4) mice stimulated with LPS (100 ng ml−1) for 24 h. (d–f) Mean level±s.d. of IL-1β and IL-18 measured from culture supernatants of (d) BMDM, (e) peritoneal macrophages or (f) splenic macrophages from POP2Tg (n=4) and LMC (n=4) mice stimulated with LPS (100 ng ml−1) for 24 h plus ATP (5 mM) for the last 30 min. (g,h) Mean level±s.d. of IL-1β, IL-18 and TNF measured from culture supernatants of BMDM from POP2Tg (n=4) and LMC (n=4) mice infected for 24 h with (g) F. tularensis LVS (MOI=100) or (h) F. novicida (MOI=100). To determine significance, Student’s t-test was used. *P<0.05.