| X μL | pEx-3XsgR-PYL114-Cas9 (1 μg) |
| 5 μL | 10X NEB CutSmart Buffer |
| 1 μL | KpnI (20 units/μL) (New England Biolab, USA) |
| 1 μL | EcoRI (20 units/μL) (New England Biolab, USA) |
| Up to 50 μL | ddH2O |
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