| 0.4 μL | Forward primer (AtU6-F-Kpn) (10 μM) |
| 0.4 μL | Reverse primer (sgR-R-EcoRI) (10 μM) |
| 2 μL | 10X Ex Taq buffer (Mg2+ plus) |
| 1.6 μL | dNTP mix (2.5 mM) |
| 0.1 μL | Ex Taq polymerase (5 units/μL) (Takara, Japan) |
| Up to 20 μL | ddH2O |
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