Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Analysis of heptamer formation by rETX and rETXF199E. MDCK cells were treated with 200 μl of toxins (4 μg/mL to 300 μg/mL) for 30 min at 37°C. Samples were solubilized, and analyzed by SDS-PAGE, and then immunoblotted with an anti-His monoclonal antibody and a HRP-coupled goat anti-mouse IgG antibody (1:50,000). The result was photographed using an AE-1000 cool CCD image analyzer.
