Fig. 4.

qPCR analysis of differentially expressed JA biosynthetic genes. Seven day-old Arabidopsis wild type (white bars) and fbs1–1 (black bars) seedlings were either untreated (time 0) or treated for up to three hours with 10 °C chilling temperature. Transcript abundances for a LOX2, b AOC1, c AOC2, d AOC3, e AOS, and f OPR1 are shown. All genes have statistically significant differences between the genotypes for at least one time point (two-way ANOVA; asterisks indicate statistical significance p < 0.05 between genotypes using Bonferroni post-tests). Shown are the average relative quantities (NRQs) (± SEM) normalized to IPP2 and PP2A within the same sample and to the wild type expression level for that gene in untreated seedlings, which was set to 1, from three independent experimental replicates