Figure 2.

Effect of purified IgG on cytokine production by intra-thymic TDP cells. Thymocytes from children less than 7 d old (n = 14) were evaluated after 3 (A-E) or 7 d (F-J) in culture in RPMI medium supplemented with FBS in the absence (mock) or presence of 100 µg/mL IgG purified from atopic or non-atopic individuals. At each time point, the frequencies of cells displaying intracellular IFN-γ, IL-17A, TNF, TGF-β and IL-10 production were evaluated by flow cytometry. The results are illustrated by box and whiskers graphs with 25th percentiles, and the Tukey method was used to plot outliers; *p ≤ 0.05 between the indicated groups.