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. 2017 Jun 30;13(6):196–201. doi: 10.6026/97320630013196

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© 2017 Biomedical Informatics

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.

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A - Toluidine blue staining of demineralized tissue sections of the paraffin embedded bone tissue following 28 days of ex-vivo culture showed positive staining indicative of collagen content; B, C - Immuno-histo-chemistry images showing positive staining for human collagen II in Group II (BM-MSCs) and Group-IV (BM-MSCs + cartilage homogenate); D - Sircol (collagen) assay showing the secreted collagen levels at days 7, 14 and 21 from various treatment groups. The values are shown as mean ± SEM from three independent samples. Statistical analysis was conducted using one-way ANOVA test. Asterisk (*) indicates statistical significance (P<0.05).