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. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: Mol Cancer Ther. 2017 Jan 30;16(4):662–670. doi: 10.1158/1535-7163.MCT-16-0526

Figure 3.

Figure 3

Delta-24-RGD (D-24-RGD) induced autophagy in pancreatic cancer cells. (A) Autophagy assay with LC3B-GFP fusion protein. Cells were infected with D-24-GDR (1MOI) for 5 days followed by infection with LC3-GFP expression baculovirus for 24 hours, and then LC3B-GFP punctae (arrow indicated) were checked under microscope. (B) Quantification of LC3B-GFP punctae. Punctae in 10 random views were counted under microscope. Results are mean +/− SD, **, p<0.01. (C) Acridine Orange staining. Cells were infected with D-24-RGD virus at different MOIs, and 5 days post infection, cells were harvested and stained with acridine orange followed by flow cytometric analysis; AVO, acidic vesicular organelle; results are mean +/− SD, *, p<0.5; **, p<0.01. (D) Western blotting assay. Cells were infected with or Ad-GFP-RGD (Ad-GFP) control or D-24-RGD for 5 days, and cell lysates were harvested with RIPA buffer for western blotting assay using the indicated antibodies.