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Lignin and lignin gene transcript localization by in situ hybridization in flax stems, roots and leaves. A set of paraffin stem sections (10 μm-thick) were first hybridized with the corresponding sense probes (left column) in order to confirm the specificity of the signal. Bar = 50 μm. The top panels show lignin localization (in red) by phloroglucinol-HCl staining. Phl I: primary phloem; xyl I: primary xylem; xyl II: secondary xylem; cz: cambial zone; bf: bast fiber. Bar = 50 μm
