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. 2017 Aug;23(8):1180–1187. doi: 10.1261/rna.061473.117

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© 2017 Nousch et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 2. — Impact of different GLD-2 mutant proteins on mRNA polyadenylation. (A) sPAT analysis of gld-1 and oma-2 mRNA in wild-type (wt) and different gld-2 backgrounds. The lane RH/dT is a wild-type sample that was treated with oligo(dT) and RNase H prior to the sPAT assay to indicate the size of a completely deadenylated mRNA. Line scans from data generated by the sPAT assay are given below. Two independent biological repeats were analyzed. (B) The distribution of bulk poly(A) tails was analyzed in wild-type and the given gld-2 backgrounds. (C) Line scans from bulk poly(A) measurements. Three independent biological repeats were analyzed. Regions of statistically significant differences indicated with a gray stripe and black bracket are calculated via the Student's t-test.