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. 2017 Aug;23(8):1180–1187. doi: 10.1261/rna.061473.117

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© 2017 Nousch et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 3. — Levels of GLD-2 target mRNAs are reduced in GLD-2(P652L)-expressing animals. (A) Abundance measurements of the indicated mRNAs via quantitative real-time PCR. Statistically significant differences are indicated and calculated via the Student's t-test. (***) P < 0.001, (**) P < 0.01, (*) P < 0.05; n.s., not significant. (B) Western blot analysis of the indicated proteins in fog-1 gld-2 double-mutant backgrounds. (C) mRNA abundance analysis during the development from L3 larvae to adults in given mutant backgrounds. L3, L3 larvae; L4, L4 larvae; earlyA, L4 + 12h; Adult, L4 + 24h.