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. 2017 Aug;23(8):1180–1187. doi: 10.1261/rna.061473.117

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© 2017 Nousch et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 4. — gld-2(q540) largely phenocopies the genetic null allele of gld-2. (A) Cartoon of the adult hermaphroditic syncytial germline tissue. Circles represent nuclei, T-structures are partial membranes. Important female germ cell stages are indicated; distal is top left, the position of sperm marks the most proximal end of the gonad. Color marked regions were analyzed in this work. (B) Analysis of the size of the proliferative region. Germ cell rows before meiotic entry were counted in the different genetic backgrounds as judged by DAPI staining. Significance was calculated by a two-tailed Student's t-test. (***) P ≤ 0.001. (C) Percentage of germlines that positively stain for diakinetic DNA in the proximal part, assessed by DAPI staining. (D–G) DAPI (purple in merge) staining of the indicated genetic backgrounds. NPC (green in merge), nuclear pore complex. Scale bar: (G) 15 µm, (blow-up) 5 µm.