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. 2017 Aug;23(8):1200–1208. doi: 10.1261/rna.058511.116

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© 2017 Lindblad et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article, published in RNA, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.

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FIGURE 3. — Template-seq reads cover entire chromosomes. While the aggregate of average read depth across all chromosomes (A) varies along the span of a chromosome, all portions are well covered, including noncoding, subtelomeric regions. There is no significant difference in the proportions of bases belonging to different sequence categories (B) between RNA-seq reads in this study and the genomic background, which indicates that template RNAs are noncoding, containing both introns and intergenic DNA, and that they can cover entire chromosomes from telomere to telomere.