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. 2017 Aug;23(8):1285–1289. doi: 10.1261/rna.061051.117

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© 2017 Loughran et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article, published in RNA, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.

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FIGURE 4. — (A) Readthrough efficiencies determined by dual luciferase assay after transfection of HEK293T cells with either pSGDluc (light gray) or pDluc (dark gray) harboring readthrough signals from human SACM1L, ACP2-1, ACP2-2, BRI3BP, MDH, LDH, and VEGFA, as indicated. Readthrough efficiency of UGA_C cloned into pSGDluc representing background levels of readthrough on UGA_C is indicated by a dashed line. (B) Predicted RNA stem–loop structure immediately 3′ of the ACP2 CDS indicating the 3′ boundaries of readthrough signals for ACP2-1 and ACP2-2. The second stop codon was mutated to UGG in construct ACP2-2. Stop codons are in red font.