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Readthrough efficiencies determined by dual luciferase assay after transfection of HEK293T cells with pSGDluc harboring readthrough signals from human SACM1L, ACP2-1, ACP2-2, BRI3BP, MDH, LDH, and VEGFA, as indicated. Normalized (Firefly/Renilla) luciferase activites were calculated for each as a percentage of their individual normalized UGG in-frame controls (light gray) or else as a percentage of a UGG_C in-frame control (dark gray).
