FIGURE 1.

Schematic overview of specific RNP capture. Specific RNP capture uses antisense LNA/DNA mixmer oligonucleotides that are covalently coupled to a magnetic resin. By applying UV crosslinking on living cells or extracts at 254 nm, direct RNA–protein interactions are covalently fixed. After cellular lysis or within the extracts, hybridization with the covalently coupled oligonucleotides is performed under stringent denaturing conditions at elevated temperatures. The RNA-bound proteins are identified by quantitative mass spectrometry in comparison to negative controls.