TABLE 1.

RBPs enriched by 28S rRNP capture

As the noCL control was devoid of proteins and cCL/noCL ratios could not be estimated, a semiquantitative approach described in Sysoev et al. (2016) was used to identify rRNA-binding proteins. Values in columns 2, 3, and 4 represent number (n) of protein identification across replicates. Proteins were classified according to functional roles in ribosome biology, including components of the cytoplasmic ribosome or implication in ribosome biogenesis reported by system-wide screens or other analyses. Three recent ribosomal biogenesis screens were taken into consideration: Wild et al. (2010) (siRNA-based biogenesis screen of the 40S and 60S ribosomal subunit using microscopy as a read-out); Tafforeau et al. (2013) (siRNA-based rRNA maturation screen using Northern blotting as a read-out); Badertscher et al. (2015) (genome-wide siRNA-based biogenesis screen of the 40S ribosomal subunit using microscopy as a read-out).