Step	Temp	Time
Initial denaturation	98°C	30 sec
One cycle	98°C	5–10 sec
	55°C	10–30 sec
	72°C	20–30 sec/kb
32 cycles	98°C	5–10 sec
	62°C	10–30 sec
	72°C	20–30 sec/kb
Final extension	72°C	2 min
Hold	4–10°C