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. Author manuscript; available in PMC: 2017 Jul 17.
Published in final edited form as: Hum Reprod. 2015 Aug 6;30(10):2351–2363. doi: 10.1093/humrep/dev194

Figure 3.

Figure 3

Expression of somatic cell markers in human fetal testis cultures. Fetal testis tissue aged between gestational week (GW) 7-9 was cultured for 2 weeks with or without presence of retinoic acid (RA) in the media and are compared to pre-culture and age-matched controls (not cultured). The age-matched control corresponds to the age of fetal samples at the time of experimental start plus two weeks. Tissue from 7 fetuses was investigated. A: Immunohistochemical staining with the Sertoli cell marker SRY (sex determining region Y)-box 9 (SOX9) and doublesex and mab-3 related transcription factor 1 (DMRT1) that stains a subpopulation of gonocytes and immature Sertoli cells in human fetal testis cultures. B: Immunohistochemical staining with the immature Sertoli cell marker anti-Müllerian hormone (AMH) and the peritubular/Leydig cell marker COUP transcription factor 2 (COUP-TFII) in human fetal testis cultures. Serial sections of tissue were used and are shown in each column. Counterstaining with Mayer haematoxylin, scale bar corresponds to 50 µm. Abbreviations: RA, retinoic acid; XX, fetal ovaries; XY, fetal testes.