Table I.
Antibodies, dilutions and retrieval buffers used.
| Antibody | Dilution | Retrieval buffer | Species | Supplier | Number |
|---|---|---|---|---|---|
| OCT4 | 1:50 | TEG | Mouse | Santa Cruz | Sc-5279 |
| AP2γ | 1:40 | Urea | Mouse | Santa Cruz | Sc-12762 |
| AMH | 1:150 | Urea | Mouse | Gift from R. Cate | - |
| SOX9 | 1:400 | CIT | Rabbit | Millipore | AB5535 |
| COUP-TFII | 1:75 | CIT | Mouse | Perseus Proteomics | PP-H7147-60 |
| FOXL2 | 1:75 | CIT | Rabbit | Gift from D. Wilhelm | - |
| DMRT1 | 1:400 | CIT | Rabbit | Sigma | HPA027859 |
| MAGE-A4 | 1:250 | TEG | Mouse | Gift from G. Spagnoli | - |
| γH2AX | 1:800 | TEG | Mouse | Abcam | Ab26350 |
| Ki-67 | 1:75 | TEG | Mouse | Dako | M7240 |
| BrdU | 1:100 | CIT | Mouse | Dako | M0744 |
| cCaspase 3 | 1:200 | CIT | Rabbit | Abcam | Ab-2302 |
| cPARP | 1:75 | CIT | Rabbit | Cell Signaling | 5525 |
For all antibodies, antigen retrieval was conducted by microwaving the sections in indicated retrieval buffer for 15 min. Citrate (CIT) buffer: 10 mM, pH 6.0; TEG buffer: 10 mM Tris, 0.5 mM EGTA, pH 9.0; Urea buffer: 5% w/v carbamide, pH 5.5. Abbreviations: cCaspase 3, cleaved caspase 3; cPARP, cleaved PARP.