Table 1.
Strains and plasmids used in the study
| Strain or plasmid | Characteristics | References |
|---|---|---|
| Streptococcus suis | ||
| SC84 | Serotype 2 strain isolated from a patient with streptococcal toxic shock-like syndrome in China | [47] |
| 1135776 | Serotype 9 strain isolated from pig following sudden death in Canada | This study |
| S2ΔagI/II | SC84-derived strain carrying an in-frame deletion of the agI/II gene | This study |
| S9ΔagI/II | 1135776-derived strain carrying an in-frame deletion of the agI/II gene | This study |
| S2CΔagI/II | SC84-derived strain carrying pOri23-S2agI/II | This study |
| S9CΔagI/II | 1135776-derived strain carrying pOri23-S9agI/II | This study |
| Escherichia coli | ||
| TOP10 | Host for pCR2.1 and pSET4s derivatives | Invitrogen |
| MC1061 | Host for pOri23 derivatives | [16] |
| BL21(DE3) | Host for pET151 derivatives | Invitrogen |
| Plasmids | ||
| pET151 | Apr, pBR322 ori, T7 promotor | Invitrogen |
| pCR2.1 | Apr, Kmr, pUC ori, lacZΔM15 | Invitrogen |
| pSET4s | Spcr, pUC ori, thermosensitive pG+host3 ori, lacZΔM15 | [37] |
| pOri23 | Ermr, ColE1 ori, P23 | [28] |
| pET151–S2agI/II | pET151 carrying the S2 agI/II gene | This study |
| pSET4s–S2agI/II | pSET4s carrying regions upstream and downstream of the S2 agI/II gene | This study |
| pSET4s–S9agI/II | pSET4s carrying regions upstream and downstream of the S9 agI/II gene | This study |
| pOri23spc–S2agI/II | pOri23 carrying the S2 agI/II gene as well as its promotor and terminator | This study |
| pOri23spc–S9agI/II | pOri23 carrying the S9 agI/II gene as well as its promotor and terminator | This study |