Table 1.
Sequences of oligonucleotide primers and details of polymerase chain reactions
| mRNA | Primers (5’→3’) F: Forward R: Reverse |
Bases in base pairs | Product size | Cycles profile Denaturation/annealing/extension (Temperature and time in seconds) |
Cycles number |
|---|---|---|---|---|---|
| β-actin | F: AGGGAAATCGTGCGTGACAT | 694 713 | 150 | 95°C / 95°C / 60°C | 40 |
| R: GAACCGCTCATTGCCGATAG | 824-843 | (20s / 3s / 30s) | |||
| VEGF | F: GCGGGCTGCTGCAATG | 1262-1277 | 268 | 95°C / 95°C / 60°C | 40 |
| R: TGCAACGCGAGTCTGTGTTT | 1528-1547 | (20s / 3s / 30s) | |||
| HIF-1α | F: ACAGCTCCCCAGCATTTCAC | 2748-2767 | 90 | 95°C / 95°C / 60°C | 40 |
| R: GGACAAACTCCCTCACCAAAAA | 2837-2816 | (20s / 3s / 30s) | |||
| PKM2 | F: TCTACGTGGACGATGGGCT | 833-851 | 403 | 95°C / 95°C / 60°C | 40 |
| R: AGGAAGACCTTCTCTGCCGGA | 1215-1235 | (20s / 3s / 30s) | |||
| RBP2 | F: TTGTGGTGACGTTTCCTCGT | 2093-2112 | 213 | 95°C / 95°C / 60°C | 40 |
| R: CAGCCAGCCCCACATCTAAG | 2305-2286 | (20s / 3s / 30s) |